Investigation of polysaccharide extracts from Iranian and French strains of Agaricus subrufescens against enzymes involved in Alzheimer’s disease

Resumen / abstract: 

In this work, the inhibitory activity of a wide range of polysaccharide extracts from two Iranian and French strains of Agaricus subrufescens were evaluated toward acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). Among them, two extracts S9 and S′7 obtained from Iranian and French strains under different extraction conditions showed selective AChE inhibitory activity with IC50 values of 154.63 and 145.43 μg/mL, respectively. It should be noted that all extracts from both strains demonstrated no BChE inhibitory activity. S9 and S′7 were also tested for their effect on amyloid beta (Aβ) aggregation, antioxidant activity, and neuroprotectivity. Their activity against Aβ aggregation was comparable to that of donepezil as the reference drug but they induced moderate antioxidant activity by DPPH radical scavenging activity and negligible neuroprotectivity against Aβ-induced damage.

Keywords: A. subrufescens; Alzheimer’s disease; Amyloid beta (Aβ); Antioxidant activity; Polysaccharide; Neuroprotectivity.

Investigación de extractos de polisacáridos de cepas iraníes y francesas de Agaricus subrufescens contra enzimas relevantes para la enfermedad de Alzheimer

 polisacáridos de dos cepas iraníes y francesas de Agaricus subrufescens. Los extractos más potentes mostraron valores de IC50 de 154,63 y 145 μg/ml para las cepas iraní (S9) y francesa (S′7), respectivamente, las cuales se obtuvieron de diferentes condiciones de extracción; sin embargo, todos los extractos no mostraron actividad inhibitoria de BChE. Además, S9 y S′7 se probaron para determinar su efecto sobre la agregación de beta-amiloide (Aβ), así como su actividad antioxidante y neuroprotectora. Su actividad inhibitoria de la agregación de Aβ fue comparable con donepezil, fármaco de referencia, pero indujeron una actividad antioxidante moderada, medida mediante la captación de radicales DPPH, y una neuroprotectora insignificante contra el daño inducido por Aβ.

Palabras clave: A. subrufescens; Enfermedad de Alzheimer; Beta amiloide (Aβ); Actividad antioxidante; Polisacarido; Neuroprotección.