Composición química de los aceites esenciales de Piper stylosum Miq. y Piper ribosoides Wall. y sus actividades antioxidantes, antimicrobiana y de inhibición de la tirosinasa.

Resumen / abstract: 

Se determinaron las composiciones químicas, las actividades antioxidante y antimicrobiana, y el contenido total de fenoles de los aceites esenciales de P. stylosum y P. ribesioides. El análisis GC y GC-MS de los aceites esenciaales de hojas y tallos de P. stylosum permitió la identificación de 50 (89.2%) y 45 (88.8%) de components, respectivamente. Los principales componentes fueron aromadendreno (hojas 26.6%; tallos 18.8%), sabineno (hojas 13.8%; tallos 6.7%) y β-cariofileno (hoja 11.5%; tallo 17.9%). Se identificaron 60 (87.0%) y 39 (82.9%) components en los aceites esenciales de hojas y tallos de P. ribesioides. Los componentes más abundantes fueron β-cariofileno (hojas 20.0%; tallos 14.4%), canfeno (hojas 16.3%; tallos 12.3%) y δ-cadineno (hojas 4.4%; tallos 7.8%). Los aceites esenciales se ensayaron para determinar sus actividades antioxidantes con DPPH y el contenido de fenoles totales. Para los aceites esenciales obtenidos se determinaron valores bajos en la actividad antioxidante con DPPH y el contenido total de fenoles, en comparación con BHT. Sin embargo, los ensayos de inhibición de tirosinasa most raron que el aceite esencial de las hojas de P. ribesioides presento la mayor inhibición (30.0%), aunque mas baja que el compuesto control (Àcido Kójico, 81.8%). Para el aceite esencial de P. ribesioides se determinó una MIC 62.5 mg/mL contra Bacillus cereus y Staphylococcus aureus.

 

Chemical composition of Piper stylosum Miq. and Piper ribesioides Wall.essential oils, and their antioxidant, antimicrobial and tyrosinaseinhibition activities.

Chemical compositions of P. stylosum and P. ribesioides essential oils, and their antioxidant, antimicrobial and tyrosinase inhibition activities were determined. GC and GC–MS analysis of essential oils from leaves and stems of P. stylosum resulted in the identification of 50 (89.2%) and 45 (88.8%) components, respectively. The major components were aromadendrene (leaves 26.6%; stems 18.8%), sabinene (leaves 13.8%; stems 6.7%) and β-caryophyllene (leaves 11.5%; stems 17.9%). A total of 60 (87.0%) and 39 (82.9%) components were identified from leaves and stems of P. ribesioides, respectively. The most abundant components were β-caryophyllene (leaves 20.0%; stems 14.4%), camphene (leaves 16.3%; stems 12.3%) and δ-cadinene (leaves 4.4%; stems 7.8%). Antioxidant activity using DPPH and total phenolic content were tested for essential oils. However, the essential oils showed low antioxidant activity and phenolic content, compared to BHT. Studies of tyrosinase inhibition showed that the essential oils of P. ribesioides leaves had the highest inhibition (30.0%), although were lower than the control (kojic acid 81.8%). The evaluation of antimicrobial activities revealed that P. ribesioides essential oils showed strong activity against Bacillus cereus and Staphylococcus aureus, both with MIC value 62.5 μg/mL.

Keywords: Essential oil, Piper stylosum, Piper ribesioides, Antioxidant, Antimicrobial, Tyrosinase inhibition