Actividad tripanocida y antibacteriana de extractos de Alvaradoa subovata Cronquist.

Resumen / abstract: 

En el presente trabajo se estudió la actividad antioxidante, antibacteriana y tripanocida de extractos de Alvaradoa subovata. La mayor actividad depuradora de radicales libres se observó en el extracto etanólico de corteza (CI50 = 4.7 ± 0.18 μg/mL). El extracto en diclorometano de madera inhibió el crecimiento de la bacteria fitopatógena Xanthomona axonopodis con una CIM = 100 μg/mL. El mismo extracto mostró inhibición del crecimiento de Trypanosoma cruzi (CI50 = 0.063 ± 0.003 mg/mL). Los resultados de este trabajo muestran que los extractos de A. subovata constituyen una fuente de compuestos bioactivos con potencial como antiparasitarios y plaguicidas.

 

Tripanocide and antibacterial activity of Alvaradoa subovata Cronquist extracts.

We studied antioxidant, antibacterial and tripanocide activities of Alvaradoa subovata extracts. The ethanolic extracts showed the greatest DPPH radical scavenging capacity, especially that of bark with an IC50 = 4.7 ± 0.18 μg/mL. Wood dichloromethane extract displayed growth inhibition of the phytopathogenic bacteria Xanthomona axonopodis in the disk diffusion assay and showed a MIC value of 100 μg/ml. It also showed growth inhibition of Trypanosoma cruzi (IC50 = 0.063 ± 0.003 mg/mL). A fraction of this extract, which has emodin as the main component, showed tripanocide activity (60% of growth inhibition at 100 ug/mL). The main compounds in wood dichloromethane extract were anthraquinones, identified as chrysophanol and emodin, and coumarins, of which scopoletin was identified. These three compound s could serve as analytical markers of the extract. The results of this study show that wood extract of A. subovata constitute a source of bioactive compounds such as antiparasitic and pesticides agents.

Keywords: Alvaradoa subovata, DPPH, Picramniaceae, tripanocide, Xanthomona.