Actividad antioxidante y antihiperglucemiante de la especie medicinal Oreocallis grandiflora (Lam.) R. Br., al sur del Ecuador.

Resumen / abstract: 

En el presente trabajo se evaluó la actividad antioxidante y antihiperglucemiante in vitro de los extractos obtenidos con los solventes: hexano, acetato de etilo y metanol, de la planta medicinal Oreocallis grandiflora (cucharillo), recolectada en la comunidad indígena de Saraguro en la provincia de Loja, al sur del Ecuador. La actividad antioxidante fue evaluada a través de los ensayos: DPPH, FOLIN-CIOCALTEU y β-CLAMS, mientras que la actividad antihiperglucemiante fue determinada por el ensayo de inhibición de α- amilasa y α-glucosidasa. El extracto metanólico de Oreocallis grandiflora presenta efecto inhibitorio sobre la enzima α-amilasa, su concentración inhibitoria (CI50) es de 109 μg/ml, frente a 126 μg/ml del control positivo Glucobay®. Además, muestra efecto inhibitorio sobre la enzima α-glucosidasa, su concentración inhibitoria (CI50) es de 3 μg/ml, frente a 1316 μg/ml del Glucobay®. Muestra también actividad antioxidante, su concentración inhibitoria (CI50) es de 15 μg/ml, frente a 5 μg/ml del α-tocoferol.

 

Antioxidant and antihyperglycemic activity of the medicinal specie Oreocallis grandiflora (Lam.) R. Br., in southern Ecuador.

In this study we evaluated the antioxidant and antihyperglycemic activity in vitro of the extracts obtained with solvents: hexane, ethyl acetate and methanol, of the medicinal plant Oreocallis grandiflora (cucharillo), collected in the Saraguro indian community of the province Loja, southern Ecuador. The antioxidant activity was evaluated by the tests: DPPH, FOLIN-CIOCALTEU and β-CLAMS, while the antihyperglycemic activity was determined by inhibition assay α-amylase and α-glucosidase. The samples were diluted to different concentrations and the reading was performed in a UV spectrophotometer, using as positive control α-tocopherol for DPPH and Folin-ciocalteu test, trolox for β-CLAMS test, and Glucobay® for testing α-amylase and α-glucosidase.The results are expressed as IC50, these show that the methanol extract of Oreocallis grandiflora has inhibitory effect on α-amylase, the IC50 is 109 μg/ml, compared to 126 μg/ ml of Glucobay®. It also shows inhibitory effect on α-glucosidase, the IC50 is 3 μg/ml compared to 1316 μg/ml of Glucobay®. It also shows antioxidant activity, its IC50 is 15 μg/ml compared to 5 μg/ml of α-tocopherol.

Keywords: medicinal plant, antioxidant, antihyperglycemic, α-tocopherol, DPPH, Folin-ciocalteu, trolox, Glucobay®, α-amylase and α-glucosidase.